Molecular Imaging Probes Based on Bioluminescence and Fluorescence
نویسندگان
چکیده
منابع مشابه
Molecular probes for fluorescence lifetime imaging.
Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of fluorescence intensity signals from fluorophores or their bioconjugates. To overcome the concentration dependency of intensity measurements, evaluate subtle molecular interactions, and determine biochemical status of intracellular or extracellular microenvironments, flu...
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Förster resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) are two major biophysical techniques for studying nanometerscale motion dynamics within living cells. Both techniques read photoemission from the transient RET-excited acceptor, which makes RET and detection processes inseparable. We here report a novel hybrid strategy, bioluminescence assisted switchi...
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Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of molecular imaging in biological research. Small animal imaging with bioluminescence has been validated recently in a variety of research models and have been shown to provide continuous quantitative monitoring of the location(s), magnitude, and time-variation of gene delivery and/or expre...
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Fluorescence lifetime (FLT) properties of organic molecules provide a new reporting strategy for molecular imaging in the near infrared (NIR) spectral region. Unfortunately, most of the NIR fluorescent dyes have short FLT typically clustered below 1.5 ns. In this study, we demonstrate that a new class of NIR fluorescent dyes, pyrrolopyrrole cyanine dyes, have exceptionally long FLTs ranging fro...
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Optical imaging of whole, living animals has proven to be a powerful tool in multiple areas of preclinical research and has allowed noninvasive monitoring of immune responses, tumor and pathogen growth, and treatment responses in longitudinal studies. However, fluorescence-based studies in animals are challenging because tissue absorbs and autofluoresces strongly in the visible light spectrum. ...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2009
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.58.435